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      Raw data and analysis for paper "Identification of polymer surface adsorbed proteins implicated in pluripotent human embryonic stem cell expansion"

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      Read this first (1).docx (13.32Kb)
      Cell and protein data.xlsx (544.9Kb)
      Primary Screen.zip (4.212Gb)
      Secondary Screen.zip (4.826Gb)
      13-10-14 Proteins from PE and CM Bovine analysis proteins.xlsx (140.3Kb)
      graphic table of content.tif (2.761Mb)
      Publication date
      2016-07-21
      Creators
      Hammad, Moamen
      Smith, James
      Rao, Wei
      Metadata
      Show full item record
      Description
      The two aims 1) identification of protein uniquely adsorbed to plasma etched tissue culture polystyrene using proteomics; 2) quantify and analyse pluripotent cell adherence to a range of arrayed micro environments on N-(4-hydroxyphenyl)methacryalamide. Purpose to identify novel protein pretreatments using high throughput screening. Previous publications have shown human pluripotent adherence to the plasma etched tissue culture polystyrene and N-(4-hydroxyphenyl)methacryalamide.
      External URI
      • https://rdmc.nottingham.ac.uk/handle/internal/52
      DOI
      • http://doi.org/10.17639/nott.49
      Subjects
      • Protein engineering
      • Proteomics
      • Cell adhesion
      • Tissue culture
      • Protein adsorption; proteomics; pluripotent cell adherence; protein pretreatments; N-(4-hydroxyphenyl)methacryalamide; plasma etched tissue culture polystyrene; high throughput screening; heat shock proteins
      • Biological Sciences::Molecular biology, biophysics & biochemistry::Biomolecular science
      • Q Science::QH Natural history. Biology
      • Q Science::QR Microbiology
      Divisions
      • University of Nottingham, UK Campus::Faculty of Science::School of Pharmacy
      Deposit date
      2016-07-22
      Data type
      Word files; Tif files; Excel files
      Funders
      • Engineering & Physical Sciences Research Council
      Grant number
      • EP/H045384/1
      Collection dates
      • May 2014-July 2014
      Data collection method
      An Ix51 IMSTAR microscope was used for all image acquisition. The IMSTAR microscope is equipped with an automated stage making it suitable for investigating large numbers of arrayed materials on a single slide. Acquisition was achieved using the in-built software; by generating a position list to image each arrayed spot or imaging an operator defined area. For the primary screening array a position list of 36 x 92 positions was generated and captured. For the secondary screening array a position list of 35 x 105 positions was generated and captured. Each position represents one polymer spot. Focus was calculated based on operator assigned landmarks on the area extremities. All images were captured using a ProgRes MF (Jenoptik) monochrome CCD digital camera.
      Resource languages
      • en
      Publisher
      University of Nottingham

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