Raw data and analysis for paper "Identification of polymer surface adsorbed proteins implicated in pluripotent human embryonic stem cell expansion"
| dc.contributor.author | Hammad, Moamen | |
| dc.contributor.author | Smith, James | |
| dc.contributor.author | Rao, Wei | |
| dc.date.accessioned | 2016-07-22T14:35:29Z | |
| dc.date.available | 2016-07-22T14:35:29Z | |
| dc.date.issued | 2016-07-21 | |
| dc.identifier.uri | https://rdmc.nottingham.ac.uk/handle/internal/52 | |
| dc.description.abstract | The two aims 1) identification of protein uniquely adsorbed to plasma etched tissue culture polystyrene using proteomics; 2) quantify and analyse pluripotent cell adherence to a range of arrayed micro environments on N-(4-hydroxyphenyl)methacryalamide. Purpose to identify novel protein pretreatments using high throughput screening. Previous publications have shown human pluripotent adherence to the plasma etched tissue culture polystyrene and N-(4-hydroxyphenyl)methacryalamide. | en_UK |
| dc.language.iso | en | en_UK |
| dc.publisher | University of Nottingham | en_UK |
| dc.subject.lcsh | Protein engineering | en_UK |
| dc.subject.lcsh | Proteomics | en_UK |
| dc.subject.lcsh | Cell adhesion | en_UK |
| dc.subject.lcsh | Tissue culture | en_UK |
| dc.title | Raw data and analysis for paper "Identification of polymer surface adsorbed proteins implicated in pluripotent human embryonic stem cell expansion" | en_UK |
| dc.type | dataset | |
| dc.identifier.doi | http://doi.org/10.17639/nott.49 | |
| dc.subject.free | Protein adsorption; proteomics; pluripotent cell adherence; protein pretreatments; N-(4-hydroxyphenyl)methacryalamide; plasma etched tissue culture polystyrene; high throughput screening; heat shock proteins | en_UK |
| dc.subject.jacs | Biological Sciences::Molecular biology, biophysics & biochemistry::Biomolecular science | en_UK |
| dc.subject.lc | Q Science::QH Natural history. Biology | en_UK |
| dc.subject.lc | Q Science::QR Microbiology | en_UK |
| dc.date.collection | May 2014-July 2014 | en_UK |
| uon.division | University of Nottingham, UK Campus::Faculty of Science::School of Pharmacy | en_UK |
| uon.funder.controlled | Engineering & Physical Sciences Research Council | en_UK |
| uon.datatype | Word files; Tif files; Excel files | en_UK |
| uon.grant | EP/H045384/1 | en_UK |
| uon.collectionmethod | An Ix51 IMSTAR microscope was used for all image acquisition. The IMSTAR microscope is equipped with an automated stage making it suitable for investigating large numbers of arrayed materials on a single slide. Acquisition was achieved using the in-built software; by generating a position list to image each arrayed spot or imaging an operator defined area. For the primary screening array a position list of 36 x 92 positions was generated and captured. For the secondary screening array a position list of 35 x 105 positions was generated and captured. Each position represents one polymer spot. Focus was calculated based on operator assigned landmarks on the area extremities. All images were captured using a ProgRes MF (Jenoptik) monochrome CCD digital camera. | en_UK |
